Lab Safety and Ethics - Revision history

Lxpk at 15:15, 24 December 2021

2021-12-24T15:15:57Z

← Older revision		Revision as of 08:15, 24 December 2021
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	{{safety}}		{{safety}}
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			'''With great power comes great responsibility.''' Noisebridge has some powerful tools and technologies being used with big machines and lasers and the like comes the need to take reasonable precautions to protect everyone's safety and ensure we aren't the baddies.
			{{boxend}}

	==General Safety Guidelines==		==General Safety Guidelines==

Lxpk: Lxpk moved page Noisebridge Lab Safety and Ethics Page to Lab Safety and Ethics: Noisebridge and Page are redundant words in page names on Noisebridge wiki

2021-12-08T20:21:06Z

Lxpk moved page Noisebridge Lab Safety and Ethics Page to Lab Safety and Ethics: Noisebridge and Page are redundant words in page names on Noisebridge wiki

← Older revision	Revision as of 13:21, 8 December 2021
(No difference)

Lxpk at 20:19, 8 December 2021

2021-12-08T20:19:51Z

← Older revision		Revision as of 13:19, 8 December 2021
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			{{safety}}

	==General Safety Guidelines==		==General Safety Guidelines==

SpammerHellDontDelete: /* General Safety Guidelines */ fix typo

2010-08-15T12:37:47Z

General Safety Guidelines: fix typo

← Older revision		Revision as of 05:37, 15 August 2010
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	* Many materials used in wet biology are flammable or otherwise harmful and will be kept in a locked cabinet labeled with the appropriate warning labels (ie: flammable, skull and crossbones, etc.). Flammables, acids, and bases should be stored in a segregated manner.		* Many materials used in wet biology are flammable or otherwise harmful and will be kept in a locked cabinet labeled with the appropriate warning labels (ie: flammable, skull and crossbones, etc.). Flammables, acids, and bases should be stored in a segregated manner.

	* Safety ~~Material~~ Data Sheets will be kept near the cabinet for all materials stored there.		* Material Safety Data Sheets will be kept near the cabinet for all materials stored there.

	* Noisebridge is a humane space -- no animal experimentation!		* Noisebridge is a humane space -- no animal experimentation!

SpammerHellDontDelete: /* General Safety Guidelines */

2009-08-13T07:40:45Z

General Safety Guidelines

← Older revision		Revision as of 00:40, 13 August 2009
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	* Noisebridge is a humane space -- no animal experimentation!		* Noisebridge is a humane space -- no animal experimentation!

	* '''Extra caution must be used in dealing with chemicals and cell cultures for not just healthy adults, but also children, elderly, immunosuppressed people, people with high allergen reactions, pets, and anything the pets may ingest including roaches/mice/pests that may reside at Noisebridge. All of these things come into Noisebridge and we can by NO MEANS be thought of as lab environment (until we come up with dedicated space or that).'''		* '''Extra caution must be used in dealing with chemicals and cell cultures for not just healthy adults, but also children, elderly, immunosuppressed people, people with high allergen reactions, pets, and anything the pets may ingest including roaches/mice/pests that may reside at Noisebridge. All of these things come into Noisebridge and we can by NO MEANS be thought of as lab environment (until we come up with dedicated space for that).'''

	* ''Know your materials, know your materials, know your materials''		* ''Know your materials, know your materials, know your materials''

Jperfetto: /* Liquid Bleach Sterilization */

2009-03-10T01:00:12Z

Liquid Bleach Sterilization

← Older revision		Revision as of 18:00, 9 March 2009
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	==== Liquid Bleach Sterilization ====		==== Liquid Bleach Sterilization ====
	Liquid solutions may be sterilized adding 10% bleach to the solution and letting sit for 20 minutes. Following sterilization, liquid waste may be poured down the drain.		Liquid solutions may be sterilized by adding 10% bleach to the solution and letting sit for 20 minutes. Following sterilization, liquid waste may be poured down the drain.

	=== Chemical Waste ===		=== Chemical Waste ===
	Noisebridge currently does not have the permits or processes necessary to handle hazardous waste generation and disposal. Therefore no chemicals or reagents requiring hazardous waste disposal should be brought into Noisebridge until such processes are setup.		Noisebridge currently does not have the permits or processes necessary to handle hazardous waste generation and disposal. Therefore no chemicals or reagents requiring hazardous waste disposal should be brought into Noisebridge until such processes are setup.

Jperfetto at 00:39, 10 March 2009

2009-03-10T00:39:52Z

← Older revision		Revision as of 17:39, 9 March 2009
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	All waste that has come in contact with bacteria, yeast, or transgenic cells is considered biohazard waste. Biohazard waste must be sterilized before being disposed of. The following two methods of sterilization may be used:		All waste that has come in contact with bacteria, yeast, or transgenic cells is considered biohazard waste. Biohazard waste must be sterilized before being disposed of. The following two methods of sterilization may be used:

	==== Pressure Cooker ====		==== Pressure Cooker Sterilization ====
	The pressure cooker may be used to sterilize liquid and solid waste, as well as to sterilize media, glassware, tools etc. The procedures for use are as follows:		The pressure cooker may be used to sterilize liquid and solid waste, as well as to sterilize media, glassware, tools etc. The procedures for use are as follows:

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	Following sterilization, solid waste bags may be disposed of in regular trash, and liquid waste may be poured down the drain.		Following sterilization, solid waste bags may be disposed of in regular trash, and liquid waste may be poured down the drain.

	==== Liquid Bleach ====		==== Liquid Bleach Sterilization ====
	Liquid solutions may be sterilized adding 10% bleach to the solution and letting sit for 20 minutes. Following sterilization, liquid waste may be poured down the drain.		Liquid solutions may be sterilized adding 10% bleach to the solution and letting sit for 20 minutes. Following sterilization, liquid waste may be poured down the drain.

	=== Chemical Waste ===		=== Chemical Waste ===
	Noisebridge currently does not have the permits or processes necessary to handle hazardous waste generation and disposal. Therefore no chemicals or reagents requiring hazardous waste disposal should be brought into Noisebridge until such processes are setup.		Noisebridge currently does not have the permits or processes necessary to handle hazardous waste generation and disposal. Therefore no chemicals or reagents requiring hazardous waste disposal should be brought into Noisebridge until such processes are setup.

Jperfetto: Fix contents

2009-03-10T00:37:59Z

Fix contents

← Older revision		Revision as of 17:37, 9 March 2009
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	~~'''~~General Safety Guidelines		==General Safety Guidelines==
	~~'''~~
	* No experimenting with or on any surface or plate/cup/container that can hold food.		* No experimenting with or on any surface or plate/cup/container that can hold food.

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	* ''Know your materials, know your materials, know your materials''		* ''Know your materials, know your materials, know your materials''


	== Waste Disposal ==		== Waste Disposal ==

Jperfetto at 00:33, 10 March 2009

2009-03-10T00:33:31Z

← Older revision		Revision as of 17:33, 9 March 2009
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	* Don’t place your experiments near any food or drink.		* Don’t place your experiments near any food or drink.

	* Sterilize all ~~glassware~~ and ~~tools that have come in contact with bacteria or yeast once you are done with them. Sterilize~~ all waste ~~that has come into contact with bacteria or yeast prior to disposing of it.~~		* Sterilize all waste as necessary, and dispose of all waste properly. Specific guidelines for waste disposal may be found below.

	* No flushing cells/cultures down the toilet or generally disposing of anything that has touched the cells without thorough disinfecting it with bleach or Lysol first or sterilizing it in the pressure cooker. ~~Certain cultures, like E. Coli have an awful smell to them, and can~~ be ~~quite difficult to get rid of~~.

	* Disinfect/wipe down of any surface you've been working on with Lysol or ethanol.		* Disinfect/wipe down of any surface you've been working on with Lysol or ethanol.
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	* ''Know your materials, know your materials, know your materials''		* ''Know your materials, know your materials, know your materials''

	== ~~Sterilization Guidelines~~ ==
	=== Pressure Cooker ===		== Waste Disposal ==
			=== Biohazard Waste ===
			All waste that has come in contact with bacteria, yeast, or transgenic cells is considered biohazard waste. Biohazard waste must be sterilized before being disposed of. The following two methods of sterilization may be used:

			==== Pressure Cooker ====
	The pressure cooker may be used to sterilize liquid and solid waste, as well as to sterilize media, glassware, tools etc. The procedures for use are as follows:		The pressure cooker may be used to sterilize liquid and solid waste, as well as to sterilize media, glassware, tools etc. The procedures for use are as follows:

			* Solid waste should be put inside an autoclave-safe bag. The bag should not be completely closed to allow steam to enter. A piece of pressure-sensitive autoclave tape should be placed outside the bag.
	* The pressure cooker should obtain a temperature of 121C and maintain this temperature for 20 minutes. When sterilizing larger quantities of media (i.e. 1 L) a longer time of 30 minutes is required.		* The pressure cooker should obtain a temperature of 121C and maintain this temperature for 20 minutes. When sterilizing larger quantities of media (i.e. 1 L) a longer time of 30 minutes is required.
	* Pressure-sensitive autoclave tape should be used on media, glassware, and waste to ensure proper sterilization.		* Pressure-sensitive autoclave tape should be used on media, glassware, and waste to ensure proper sterilization.
			''Specific instructions on using the pressure cooker should be placed here once a pressure cooker has been obtained''

			Following sterilization, solid waste bags may be disposed of in regular trash, and liquid waste may be poured down the drain.

			==== Liquid Bleach ====
			Liquid solutions may be sterilized adding 10% bleach to the solution and letting sit for 20 minutes. Following sterilization, liquid waste may be poured down the drain.

	=== ~~Liquid Bleach~~ ===		=== Chemical Waste ===
	~~Liquid solutions may be sterilized adding 10% bleach~~ to ~~the solution~~ and ~~letting sit for 20 minutes~~.		Noisebridge currently does not have the permits or processes necessary to handle hazardous waste generation and disposal. Therefore no chemicals or reagents requiring hazardous waste disposal should be brought into Noisebridge until such processes are setup.

Jperfetto at 00:06, 10 March 2009

2009-03-10T00:06:42Z

← Older revision		Revision as of 17:06, 9 March 2009
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	* No experimenting with or on any surface or plate/cup/container that can hold food.		* No experimenting with or on any surface or plate/cup/container that can hold food.

	* Experiments ~~will have~~ their own fridge. Do not place your experiments in the food fridge.		* Experiments and reagents should be stored in their own fridge. Do not place your experiments, media, or chemicals in the food fridge.

	* Don’t place your experiments near any food or drink.		* Don’t place your experiments near any food or drink.

	* ~~Use the pressure cooker to sterilize~~ all glassware~~, media~~ and tools. ~~Water should get~~ to ~~121C or above for sterilization~~.		* Sterilize all glassware and tools that have come in contact with bacteria or yeast once you are done with them. Sterilize all waste that has come into contact with bacteria or yeast prior to disposing of it.

	* No flushing cells/cultures down the toilet or generally disposing of anything that has touched the cells without thorough disinfecting it with bleach or Lysol first or sterilizing it in the pressure cooker. Certain cultures, like E. Coli have an awful smell to them, and can be quite difficult to get rid of.		* No flushing cells/cultures down the toilet or generally disposing of anything that has touched the cells without thorough disinfecting it with bleach or Lysol first or sterilizing it in the pressure cooker. Certain cultures, like E. Coli have an awful smell to them, and can be quite difficult to get rid of.

	* Disinfect/wipe down of any surface you've been working on with ~~bleach~~ or ~~Lysol~~.		* Disinfect/wipe down of any surface you've been working on with Lysol or ethanol.

	* Cover dishes if you aren't directly working with your culture (to keep from contaminating your culture, your peers and Noisebridge).		* Cover dishes if you aren't directly working with your culture (to keep from contaminating your culture, your peers and Noisebridge).

	* Know how to safely use and dispose of materials (reagents for example) before they enter Noisebridge.		* Know how to safely use and dispose of materials (reagents for example) before they enter Noisebridge. Do not bring reagents in which require hazardous waste disposal - Noisebridge currently does not have the permits or processes necessary for this.

	* Many materials used in wet biology are flammable or otherwise harmful and will be kept in a locked cabinet labeled with the appropriate warning labels (ie: flammable, skull and crossbones, etc.)		* Many materials used in wet biology are flammable or otherwise harmful and will be kept in a locked cabinet labeled with the appropriate warning labels (ie: flammable, skull and crossbones, etc.). Flammables, acids, and bases should be stored in a segregated manner.

	* Safety Material Data Sheets will be kept near the cabinet for all materials stored there.		* Safety Material Data Sheets will be kept near the cabinet for all materials stored there.
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	* ''Know your materials, know your materials, know your materials''		* ''Know your materials, know your materials, know your materials''

			== Sterilization Guidelines ==
			=== Pressure Cooker ===
			The pressure cooker may be used to sterilize liquid and solid waste, as well as to sterilize media, glassware, tools etc. The procedures for use are as follows:
			* The pressure cooker should obtain a temperature of 121C and maintain this temperature for 20 minutes. When sterilizing larger quantities of media (i.e. 1 L) a longer time of 30 minutes is required.
			* Pressure-sensitive autoclave tape should be used on media, glassware, and waste to ensure proper sterilization.

			=== Liquid Bleach ===
			Liquid solutions may be sterilized adding 10% bleach to the solution and letting sit for 20 minutes.